Occurs in two stages:
1) Light dependent reactions (LDR)
- photon hits chlorophyll molecule in PS II and excites electron. Photolysis of H2O into oxygen, protons and electrons is triggered
- released electrons pass through series of electron carriers before reaching PS I
- at PS I, electrons are excited again by incident photons. They pass through ferredoxin electron carrier
- Electrons can either travel back to plastoquinone, powering proton pump or reach NADP reductase enzyme where NADP+ ions are reduced to NADPH
- Proton gradient powers production of ATP from ATP-ase enzymes in thylakoid membrane
2) Light independent reactions
- rubisco enzymes catalyses the fixing of the CO2 molecule to the 5-carbon ribulose biphosphate
- decays into two 3- carbon molecules of phosphoglycerate
- ATP from LDR is used to form diphosphoglycerate
- NADPH used to reduce this to GALP
- one molecule of GALP is removed per 3 molecules of CO2 and the rest are modified into (i) ribulose phosphate and (ii) ribulose biphosphate
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